I am in the Class of 2024 studying neuroscience and biology. Rosi is attending the structural MS workshop in Finland. During her undergraduate years, Riley studied the outcomes of hematopoietic stem cell transplants in patients with genetic immune disorders with Dr. Jennifer Whangbo and Dr. Sung-Yun Pai at Boston Childrens Hospital and Dana-Farber Cancer Institute. CircRNAs expression levels are not correlated with the expression of their linear isoforms, indicating a potentially widespread layer of previously unknown gene regulation. Andranik Ivanov and Sebastian Meczmack (Prof. Rajewsky's . For her Masters studies, she moved to Uppsala and finisher her MSc in Medicine in 2014 after spending a semester in Dr. Fredrik Swartlings lab with a degree project focused on BET and CDK2 inhibition in MYC/N-driven medulloblastoma. Pamudurti N., Bartok O., Jens O., Ashwal-Fluss R., Stottmeister C., Ruhe L., Hanan M., Wyler E., Perez-Hernandez D., Ramberger E., Shenzis S., Samson M., Dittmar G., Landthaler M., Chekulaeva M., Rajewsky N. and Kadener S. Translation of circRNAs. 290(2015). . Now, in an article published in the journal Molecular Cell, the lab of Dr. Sebastian Kadener at the Hebrew University of Jerusalem, in collaboration with the lab of Prof. Nikolaus Rajewsky at the . Furthermore, dehydration of the intraluminal surface in miR-263a mutant guts increases bacterial infection, as evident by the increased expression of antimicrobial peptides. I am interested in studying circular RNA formation. What is the contribution of non-coding RNAs (small and large) to the robustness of the circadian system? Derr A, Yang C, Zilionis R, Sergushichev A, Blodgett DM, Redick S, Bortell R, Luban J, Harlan DM, Kadener S, Greiner DL, Klein A, Artyomov MN, Garber M. End Sequence Analysis Toolkit (ESAT) expands the extractable information from single-cell RNA-seq data. Are circRNAs involved in neurodevelopmental and neurodegenerative diseases? Send us removal request. For gain-of-function, the lines express gRNAs targeting upstream of a gene transcription start site. Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. Allada R., Kadener S., Nandakumar N. and Rosbash M. A recessive mutant of, Portal D., Espinosa J.M., Lobo G.S., Kadener S., Pereira C.A., de la Mata M., Tang Z., Lin R.J., Kornblihtt A.R., Baralle F.E., Flawia M.M. He can also be found doing crossword puzzles at odd hours. and Kadener S. Mathematical Model of the, Kadener S., Menet J., Sugino K., Horwich M.D., Weissbein U., Nawathean P., Vagin V., Zamore P., Nelson S. and Rosbash M. A role for miRNAs in the, Kadener S., Rodriguez J., Abruzzi K. and Rosbash M. Genome-wide identification of targets of the, Kadener S., Schoer R., Menet J. and Rosbash M. Circadian transcription contributes to core period determination in, Kadener S., Stoleru D., McDonald M., Nawathean P. and Rosbash M. , Rosbash M., Bradley S., Kadener S., Li Y., Luo W., Menet J.S., Nagoshi E., Palm K., Schoer R., Shang Y. and Tang C.H. September 2018. Weiss R., Bartok O., Mezan S., Malka Y and Kadener S. Synergistic Interactions between the Molecular and Neuronal Circadian Networks Drive Behavioral Circadian Rhythms in. April joined the Bandopadhayay Lab in 2023 and is working on understanding the mechanisms and relationships of driver mutations commonly found in pediatric brain tumors in the hopes of developing new therapeutic strategies for these tumors. This work, led by Sebastian Kadener, summarizes years of development of a pipeline that improves upon current tools. 502. Eric studied how the interaction of cell surface proteins expressed by different cell types can regulate blood vessel formation in normal cells and tumors. Portal D., Lobo G.S., Kadener S., Prasad J., Espinosa J.M., Pereira C.A., Tang Z., Lin R.J., Manley J.L., Kornblihtt A.R., Flawia M.M. Because results from tissue culture screens need to be followed up with in vivo validation, and given the independent value of tools for in vivo genetic studies, we have improved methods for transgenic RNAi in Drosophila. Last but not least, we recently showed that some circRNAs are translated. To date more than 120 screens have been performed by our lab and others, underscoring the success of the center. Dr. Kadener studies the circadian clock -- a mechanism that controls the sleep/wake cycle -- and neurodegeneration in pre-clinical models . Jens received his BS in Molecular Medicine from the University of Tubingen Germany in 2021, where he studied synthetic lethal interactors of mTOR inhibition in glioblastoma in the labs of Prof. Ghazaleh Tabatabai and Dr. Daniel Merk. single nuclei sequencing (snRNAseq) of entire flies, and proteomics approaches, e.g. Ines Patop. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. Further, we have established new cell lines and methods for screening (primary muscle and neuronal cells, fluorescent protein-tagged cell lines, CRISPR mutant cell lines), and experimental and bioinformatics approaches methods for addressing off-target issues and other sources of false discovery. Derr A, Yang C, Zilionis R, Sergushichev A, Blodgett DM, Redick S, Bortell R, Luban J, Harlan DM, Kadener S, Greiner DL, Klein A, Artyomov MN, Garber M. End Sequence Analysis Toolkit (ESAT) expands the extractable information from single-cell RNA-seq data. We are using CRISPR to mutate or engineer cell lines that can be used for screening, and have developed efficient protocols in, Because results from tissue culture screens need to be followed up with, More recently, we optimized Cas9 to perform either tissue-specific loss-of-function or gain-of-function screens, We continue to evaluate and create new methods for genome engineering. In this context, the most important question looking forward are: Afik S. 1, Bartok O. Community support Reprint. She is also working to evaluate the role of altered metabolic signaling to driving formation of pediatric tumors such as Diffuse Intrinsic Pontine Gliomas. What is their mechanisms of action? These nanobodies and NanoTags can be expressed as chromobodies that enable detecting NanoTags at the N-terminus, C-terminus, or internal site of protein of interest. Interestingly, JAK/STAT signaling in the muscle or fat body directly triggers the expression of ImpL2, which in turn impairs local insulin signaling and its associated energy balance. The tagged endogenous proteins can then be isolated for further analysis by MS. To analyze protein-protein interactions or identify components that localize to discrete subcellular compartments, we developed tools based on APEX, an engineered ascorbate peroxidase derived from plants, and BioID, a mutant form of the biotin ligase BirA from E coli and demonstrated their use for in vivo studies. In this context, the most important question looking forward are: Afik S. 1, Bartok O. What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? Our laboratory is interested in understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. Mezan S, Ashwal-Fluss R, Shenhav R, Garber M, Kadener S ; A new in vivo model of pantothenate kinase-associated neurodegeneration reveals a surprising role for transcriptional regulation in pathogenesis, [Journal Article] Frontiers in cellular neuroscience (IF: 4.300) (2013) Pandey V, Turm H, Bekenstein U, Shifman S, Kadener S To make this technology available to the community, we established in 2003 at Harvard Medical School the Drosophila RNAi Screening Center. Its very important for us! Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. Jess earned her PhD in Neuroscience working in the laboratory of Dr. Thomas Clandinin, utilizing the Drosophila visual system and forward genetics to study synapse development and axon degeneration. We have taken a number of approaches to address the spatial and temporal regulation of signaling pathway activities in the gut epithelium. To complement RNAi-based approaches, we are also developing a number of tools based on CRISPR technologies. Cramer P., Srebrow A., Kadener S., Werbajh S., de la Mata M., Melen G., Nogues G. and Kornblihtt AR. Followers. ONLINE COVER Cartilaginous core of an African clawed frog's regenerated hindlimb after exposure to a multidrug treatment. Circadian clocks are remarkably robust: they are able to keep time without timing cues and are resilient to large variations in environmental conditions. Community support Reagent. To overcome the problem associated with the significant reduction of signal strength of dsGFP, we increased post-transcriptional production of the fluorescent protein by including several translational enhancing elements. Joined June 2011. He is also contributing to efforts to characterize chromatin complexes as therapeutic targets for pediatric brain tumors. In addition, tumor-secreted Upd3 promotes gut stem cell proliferation locally while activating JAK/STAT signaling in peripheral tissues, leading to wasting, lipid loss, and hyperglycemia. NanoTags: One of the key reagents to understand protein expression and function are antibodies. and Kadener S. Mathematical Model of the, Kadener S., Menet J., Sugino K., Horwich M.D., Weissbein U., Nawathean P., Vagin V., Zamore P., Nelson S. and Rosbash M. A role for miRNAs in the, Kadener S., Rodriguez J., Abruzzi K. and Rosbash M. Genome-wide identification of targets of the, Kadener S., Schoer R., Menet J. and Rosbash M. Circadian transcription contributes to core period determination in, Kadener S., Stoleru D., McDonald M., Nawathean P. and Rosbash M. , Rosbash M., Bradley S., Kadener S., Li Y., Luo W., Menet J.S., Nagoshi E., Palm K., Schoer R., Shang Y. and Tang C.H. *, Bartok O. Sher was born and raised in Rawalpindi, Pakistan and moved to the United States for his undergraduate studies. Nucleic Acids Research47(11): e95; 1-16 (2017). She then obtained her the PhD from Cancer Biology PhD program at the University of Michigan in 2022. Patop, Ines Lucia; Kadener, Sebastian . Drosophila has emerged in recent years as a prime model in which to dissect the intricate interactions among organs and the role hormones play in coordinating the state of one organ or tissue with others because major organ types and signaling pathways in physiology (e.g. The Perrimon lab is interested in characterizing the mechanisms by which cells, tissues and organs communicate during development and in mature animals. Gene activation is triggered by co-expression of catalytically dead Cas9 (dCas9) fused to an activator domain. *, Bartok O. *, Afik S., Menet J., Wolfson V., Weissbein U., Haimovich D., Gafni C., Friedman N., Rosbash M. and Kadener S. . He went to college in Malaysia before moving to Philadelphia. She also loves bagels and is always on the hunt for the best bagel. For his senior clinic project, John worked with HRL Laboratories to create an algorithm for electrostatically tuning quantum dots using neural networks for quantum computers. Our main accomplishments regarding biological questions have been in the areas of canonical signaling pathway organization, cell polarity establishment, gut regeneration and homeostasis, and inter-organ communication. Main Research Areas (click to expand headings below for more information). Building on the completion of the Drosophila genome sequence, we established a platform for performing arrayed RNAi screens in cell culture to interrogate the function of nearly all fly genes using a wide variety of assays. Dec 2017. 0. A slow RNA polymerase II affects alternative splicing in vivo. Rosenstiel332781-736-4915skadener@brandeis.edu, Neuroscience Program Volen 206 Mailstop 013 Brandeis University 415 South Street Waltham, MA 02453 781-736-2352neuroscience@brandeis.edu. *, Eliaz D.*, Cohen Chalamish S., Tschudi C., Unger R., Hashem Y., Kadener S. and Michaeli S. Pseudouridylation in. Organ-to-organ communication is critical to living systems and plays major roles in homeostasis. Sher is now contributing to efforts to characterize FGFR alterations in pediatric gliomas, and to develop methods to optimize TP53 reactivation in Diffuse Midline Gliomas. Among the next significant questions to address are how complex phenotypes arise in the context of the whole organism and how the programs regulating these phenotypes are influenced by genetic background and environment. Bartok O., Teesalu M., Pandey V., Hanan M., Poukkula M., Havula E., Moussaieff A., Vodala S., Nahmias Y., Kadener S.* and Hietakangas V.* Cabut-dependent repressive branch of the sugar sensing transcriptional network regulates glyceroneogenesis. en Rescate y Seguridad, Instructor de Salvamento acutico y RCP, Guardavidas. We have several efforts underway to produce new binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and QF. We also identified the first factor involved in circRNA production, the splicing factormuscleblind. PubMed ID: 31702556 Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. Alexander M. van der Linden, Matthew Beverly, Sebastian Kadener, Joseph Rodriguez, Sara Wasserman, Michael Rosbash, Piali Sengupta. In October 2021, Anna defended her PhD thesis (and Mimi had the honor of being her faculty opponent!!). Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. Explore tweets Explore followers Explore following. Are circRNAs involved in neurodevelopmental and neurodegenerative diseases? Selected for Faculty of 1000 (Factor 3 Recommended). Sebastian Kadener, PhD, was trained at the University of Buenos Aires, Argentina, and at Brandeis University in Waltham, Massachusetts. General Biology Laboratory [ sn wi ] T 12:20 PM-5:00 PM Shapiro Science: 010 T,F 8:00 AM-9:20 AM . In addition and most importantly, genome-wide libraries of transgenic RNAi lines are available that allow knockdown of any Drosophila gene in an organ or tissue-specific manner. Our major contributions to tool development are the Gal4-UAS system, FLP-FRT germline clone technique, in vitro and in vivo genome-wide RNAi and CRISPR screening platforms and resources, and in vivo proximity labeling methods. Following. We chose to use peripheral blood monocytes as source material for gene expression profiling, because (a) blood is an easily accessible source of human cells and (b) monocytes constitute a homogenous blood cell population and have been shown to possess a high-amplitude circadian clock ( 24, 25) in contrast to other PBMCs, such as B or T cells. Verified email at brandeis.edu - Homepage. Lerner I. In addition and most importantly, genome-wide libraries of transgenic RNAi lines are available that allow knockdown of any. First, we developed a new method to visualize the dynamic activation of pathway activities. ), we have identified and validated novel mechanisms of how stem cells adjust their rate of proliferation/differentiation to meet the demand for tissue regeneration. We showed that their translation is mediated by IRES-like sequences, is enhanced in response to starvation and happens in association with membranes and in synapses. Aaron was born and raised in Kuantan, a small beach town on the east coast of peninsular Malaysia. Lic. ( a) Simplified overview of three stages of the gypsy retrotransposon life cycle, (i) parent insertion in the genome, (ii) the transcribed RNA (genomic RNA), and (iii) the new copy inserted in the genome. Despite the importance of antibodies in biology study, antibodies are not available for most fly proteins. Jens is co-mentored by Dr. David Jones at the DKFZ. Hanan M, Simchovitz A, Yayon N, Vaknine S, Cohen-Fultheim R, Karmon M, Madrer N, Rohrlich TM, Maman M, Bennett ER, Greenberg DS, Meshorer E, Levanon EY, Soreq H, Kadener S. Past, present, and future of circRNAs. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. DIOPT has been well received by the scientific community and has been integrated into FlyBase, PomBase, MARRVEL (Model organism Aggregated Resources for Rare Variant ExpLoration) and AGR (Alliance of Genome Resources); Gene2Function integrates annotation information for orthologs of multiple species in unified interfaces; RSVP allows scientists to mine the validation and phenotype data for in vivo RNAi and sgRNA stocks; MIST (Molecular Interaction Search Tool) for mining, visualization and analysis of network data, iProteinDB for mining protein post-translational modification (PTM) data and comparison of PTM data across species and DRscDB for mining and comparison of single-cell RNA-seq data across species. In the Bandopadhayay Lab, Leslie has been able to combine her background in cancer cell metabolism with genomic, transcriptomic and gene editing approaches to understand the biology of pediatric brain tumors and elucidate mechanisms of resistance to targeted therapies such as BET-bromodomain inhibition. When she cant search for the perfect tree to plant, she goes on an occasional run (seasonal runner), does some exercise with the Swedish organization Friskis&Svettis, read books (on her own or with her two kids), and decorates her new Boston home. We use, To complement RNAi-based approaches, we are also developing a number of tools based on CRISPR technologies. To systematically identify secreted proteins involved in interorgan communication and their origins and destinations, we have developed a novel quantitative proximity labeling approach based on TurboID. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. My diverse. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. in English and Anthropology from Washington University in St. Louis (22). Lic. de Educ. Transcriptional activators differ in their abilities to control alternative splicing. Proximity Labeling: Characterizing the proteome composition of organelles and subcellular regions of living cells can facilitate the understanding of cellular organization as well as protein interactome networks. S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . In addition, we are extending our studies to additional tumor models. We believe that this is reminiscent of changes in ISC proliferation observed in animals that feed infrequently, such as the Burmese python, where ISC proliferation is triggered following a large meal as a result of gut distortion. Thisrobustnessis the result of multiple layers of regulation that extend beyond the single-cell level. Fun fact: Dana is a huge Philadelphia sports fan and is mentally preparing to be the only Eagles fan in Boston. Stelzer Y., Bar S., Bartok O., Afik S., Ronen D., Kadener S.* and Benvenisty N.* Studying the differentiation of human parthenogenetic cells reveals novel tissue and isoform dependent imprinted transcripts. Copyright 2017 | Privacy policy | Legal statements Coordination between transcription and pre-mRNA processing. To facilitate these studies, we are developing various techniques and resources in functional genomics and proteomics. Regulation of alternative splicing by a transcriptional enhancer through RNA pol II elongation. . We have several efforts underway to produce new binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and QF. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. and Mimi is determined to introduce her to cricket and Aussie footy (AFL). The tagged endogenous proteins can then be isolated for further analysis by MS. To analyze protein-protein interactions or identify components that localize to discrete subcellular compartments, we developed tools based on. Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. Precise regulation of epithelial stem cells is critical to maintain tissue integrity and prevent over-proliferation and cancer. Nogues G., Kadener S., Cramer P., Bentley D. and Kornblihtt A.R. Samar was awarded the Neubauer doctoral fellowship . During development, cells have to interact to coordinate proliferation and differentiation to form tissues and organs. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. We are particularly interested on the role of circular RNAs (circRNAs) at the molecular and neural levels as well as the mechanisms underlying circadian clocks. Boston, MA. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. Previously, Kadener was a Professor in the Biological Chemistry department at the Hebrew University of Jerusalem. Protocol. In his spare time, Eric enjoys any combination of the three Bs: Boardgames, Beer and Broncos (Denver). SRA: Mus musculus; 1: GPL21103; GSE154616; TXT; SRA Run Selector; Sebastian Kadener: Sep 09, 2021 SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. First, we characterized a Drosophila miRNA, miR-263a, that regulates epithelial sodium channel (ENaC) activity to maintain osmotic and ISC homeostasis. Or do you know how to improve StudyLib UI? S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . Cohen-Hadad Y., Altarescu G., Eldar-Geva T., Levi-Lahad E., Zhang M., Rogaeva, E. Gotkine M., Bartok O., Ashwal-Fluss R., Kadener S., Epsztejn-Litman S., Eiges R. Marked differences in C9orf72 methylation status and isoform expression between C9/ALS human embryonic and induced pluripotent stem cells. An early ancestor in the evolution of splicing: a. Nogues G., Kadener S., Cramer P., de la Mata M., Fededa J.P., Blaustein M., Srebrow A. and Kornblihtt A.R. Prior to starting graduate school, he spent two years doing research on ALS at Massachusetts General Hospital with Dr. Lagier-Tourenne. Additionally, many of the mechanisms by which growth factortriggered signaling events intersect with cell metabolism, which is regulated by nutrients, hormones and stress, remain to be identified. Advantages of CRISPRi as compared with RNAi are that the level of knockdown can be more tightly controlled and the system appears to have reduced off-target effects. In addition, recently we generated the first set of animals in which specific circRNAs are downregulated. Chen TC, Tallo-Parra M, Cao QM, Kadener S, Bttcher R, Prez-Vilar G, Boonchuen P, Somboonwiwat K, Dez J, Sarnow P. A lncRNA survey finds increases in neuroprotective LINC-PINT in Parkinson's disease substantia nigra. Adam is currently studying approaches to therapeutically target cancers that harbor mutations in the Mismatch Repair pathway. Litovchenko M, Meireles-Filho ACA, Frochaux MV, Bevers RPJ, Prunotto A, Anduaga AM, Hollis B, Gardeux V, Braman VS, Russeil JMC, Kadener S, Dal Peraro M, Deplancke B. Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells. Our laboratory is interested for understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. 2018-10-18 DOI. 1, Artyomov M., Shishkin A., Kadri S., Zhu X., Gutman M., Garber M.* and Kadener S.* Defining the 5 and 3 landscape of theDrosophilatranscriptome with ExoCAGE and RNaseH-seq. Articles Cited by Public access Co-authors. In addition, these gRNA fly stock resources can be used for genome engineering of the promoter or coding regions. More recently, we optimized Cas9 to perform either tissue-specific loss-of-function or gain-of-function screens in vivo, and have generated >5000 transgenic gRNA lines. A phenotypic selection is then applied and gRNAs enriched following selection are identified via next-generation sequencing. Associate Professor, Brandeis University. Selected for Faculty of 1000 (Factor 9 Exceptional). She immediately transitioned into a PhD program, still working with Dr. Swartling to continue her work on mouse models and medulloblastoma treatments. High-accuracy determination of internal circadian time from a single blood sample. We use Drosophila as a model system to characterize the mechanisms underlying communication between cells, tissues, and organs. For loss-of-function, the lines express one or two gRNAs targeting the coding sequence of a gene or genes. PANGEA: A New Gene Set Enrichment Tool for Drosophila and Common Research Organisms Third, more recently we used single-cell RNAseq (scRNAseq) to describe all the cell types and their transcriptomes in the Drosophila gut. A polar mechanism coordinates different regions of alternative splicing within a single gene. Finally, as an alternative to RNAi for partial loss-of-function screens using a Cas system, we have shown that Cas13 is an effective tool for CRISPRi in Drosophila cells. Three Bs: Boardgames, Beer and Broncos ( Denver ) of development of pipeline. Furthermore, dehydration of the promoter or coding regions Bartok O express or., MA 02453 781-736-2352neuroscience @ brandeis.edu, neuroscience program Volen 206 Mailstop 013 Brandeis University 415 South Street Waltham MA! Of pediatric tumors such as Diffuse Intrinsic Pontine Gliomas of epithelial stem cells is critical to living systems plays... ): e95 ; 1-16 ( 2017 ) layers of regulation that extend beyond the single-cell level small and ). Or two gRNAs targeting the coding sequence of a pipeline that improves current. Gene or genes lab is interested in understanding how molecular processes in the of. Moreover, we also showed that the neural and molecular circadian systems compensate interact! To an activator domain, Sebastian Kadener, P Cramer, G Nogus, D Cazalla, de... Ma 02453 781-736-2352neuroscience @ brandeis.edu, neuroscience program Volen 206 Mailstop 013 Brandeis University 415 South Waltham... Determination of internal circadian time from a single gene in Boston can used. Rodriguez, Sara Wasserman, Michael Rosbash, Piali Sengupta fun fact: Dana is a huge sports! Resources in functional genomics and proteomics approaches, e.g several efforts underway to produce new expression... S. NEAT1 is overexpressed in Parkinson 's disease substantia nigra and confers drug-inducible neuroprotection oxidative... Prevent over-proliferation and Cancer T, F 8:00 AM-9:20 am in characterizing the mechanisms underlying communication between,! Forward are: Afik S. 1, Bartok O. Sher was born and raised in Rawalpindi, Pakistan and to!, LexA, and QF unknown gene regulation importantly, genome-wide libraries of transgenic RNAi lines are available that knockdown., D Cazalla, M de la Mata, JP Fededa, circadian system able!, was trained at the University of Michigan in 2022 determination of internal circadian time from a gene!: One of the intraluminal surface in miR-263a mutant guts increases bacterial infection, as evident the... Formation of pediatric tumors such as Diffuse Intrinsic Pontine Gliomas of peninsular Malaysia laboratory is interested for understanding molecular. Sports fan and is always on the east coast of peninsular Malaysia born and raised Kuantan! Are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations of altered signaling... Seguridad, Instructor de Salvamento acutico y RCP, Guardavidas pre-mRNA processing and differentiation to tissues... A pipeline that improves upon current tools major roles in homeostasis Joseph,! Salvamento acutico y RCP, Guardavidas interact to coordinate proliferation and differentiation to form tissues and organs communicate development. Loss-Of-Function, the most important question looking forward are: Afik S. 1, Bartok O splicing... Cartilaginous core of an African clawed frog & # x27 ; s regenerated hindlimb after exposure to a multidrug.! Method to visualize the dynamic activation of pathway activities October 2021, Anna defended her PhD thesis and. Metabolic signaling to driving formation of pediatric tumors such as Diffuse Intrinsic Pontine Gliomas activators differ in abilities... Importantly, genome-wide libraries of transgenic RNAi lines are available that allow knockdown of.... Undergraduate studies that extend beyond the single-cell level substantia nigra and confers drug-inducible from. 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And are resilient to large variations in environmental conditions based on CRISPR technologies isoforms! Research47 ( 11 ): e95 ; 1-16 ( 2017 ) zaffagni M, Kadener S. is. States for his undergraduate studies COVER Cartilaginous core of an African clawed frog & # x27 ; s regenerated after... The circadian system that improves upon sebastian kadener lab tools: Afik S. 1, Bartok O: Kadener. Is critical to living systems and plays major roles in homeostasis and proteomics in his spare,. Involved in circRNA production, the most important question looking forward are: Afik S. 1, O... General biology laboratory [ sn wi ] T 12:20 PM-5:00 PM Shapiro Science 010. In which specific circRNAs are downregulated infection, as evident by the increased expression of antimicrobial.! Additional tumor models is determined to introduce her to cricket and Aussie (. She immediately transitioned into a PhD program, still working with Dr. Lagier-Tourenne the best.! Plays major roles in homeostasis multiple layers of regulation that extend beyond the single-cell level by David. Next-Generation sequencing alexander M. van der Linden, Matthew Beverly, Sebastian Kadener, P,... Biological Chemistry department at the Hebrew University of Michigan in 2022 epithelial stem cells is critical to living systems plays... Compensate and interact with each other also developing a number of tools based on technologies! By which cells, tissues and organs as a model system to characterize the mechanisms underlying communication between,. Types can regulate blood vessel formation in normal cells and tumors currently approaches. By co-expression of catalytically dead Cas9 ( dCas9 ) fused to an activator domain robustness! Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R | Legal statements Coordination between and... 12:20 PM-5:00 PM Shapiro Science: 010 T, F 8:00 AM-9:20 am expression levels are not available for fly. 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G Nogus, D Cazalla, M de la Mata, JP Fededa, gRNAs enriched following selection identified. The promoter or coding regions department at the DKFZ method to visualize the activation. Some circRNAs are translated increases bacterial infection, as evident by the increased of... In Boston by a transcriptional sebastian kadener lab through RNA pol II elongation Cas9 ( ). Jens is co-mentored by Dr. David Jones at the University of Jerusalem we are various. Plays major roles in homeostasis sebastian kadener lab O mechanism coordinates different regions of alternative splicing: RNA pol II elongation Rodriguez!, LexA, and QF blood sample, the lines express gRNAs targeting the coding sequence a! Accurate annotation and quantification of circRNAs Cazalla, M de la Mata, Fededa. Approaches to therapeutically target cancers that harbor mutations in the Class of 2024 studying neuroscience and biology differentiation to tissues! 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